The use of antibiotics in the preparation of amphibian cell cultures from highly contaminated material.
نویسنده
چکیده
Embryos and larvae of Xenopus laevis are contaminated with a wide range of micro-organisms from the maternal cloaca. Primary monolayer cultures prepared from them were usually destroyed by endogenous contaminants. Fungal contamination was readily eliminated by the usual procedures. The high incidence of bacterial contamination was not reduced by penicillin, streptomycin, tetracycline or combinations of the three. Contamination by Gram-negative bacilli, especially Proteus and Pseudomonas, remained. Treatment with carbenicillin, gentamicin or the combination of polymyxin E with kanamycin usually eliminated all infection. The cytotoxicity of effective antibiotics was tested. The basic requirements of a method for eliminating endogenous bacterial contamination and the relative merits of the three effective treatments are discussed.
منابع مشابه
Antibiotic Resistance of the Isolated Bacteria from Blood Cultures of Cancer Patients
Abstract Background and Objective: One of the main causes of increased mortality in cancer patients is bacteremia. On the other hand, antibiotic resistance is the major cause of treatment failure in malignant diseases especially in hematological malignancies. The aim of this study was to diagnose the bacterial strains isolated from blood specimens of cancer patients and to determine their antib...
متن کاملPreparation and characterization of a soluble precipitating antigen from African horsesickness virus propagated in cell cultures
متن کامل
جداسازی لیستریا مونوسیتوژن از پنیر با استفاده از روش غنی سازی در سرما و مشاهده باکتری در کشت سلولی هلا
Background: Listeria monocytogenes is frequently transmitted from contaminated food such as cheese, raw vegetables, prepacked salad and etc, to humans. This bacteria can grow inter and extracellular. The aim of this study was to present a simple practical method for isolation of this bacteria from cheese, and detection of it in Hela cell culture by light microscopy Materials and Methods: In ...
متن کاملگندزدایی آبهای آلوده به کلیفرم مدفوعی با استفاده از ازن: بررسی تاثیر غلظت ازن و زمان تماس
Introduction & Purpose:Chlorine is the most important matter to water disinfection due to a residual effect. But by formation of by-products could be harmful to consumers’ health ,The researchers decided to use the method or other material. For this reason, in this study ozone used to water disinfection and its performance to disinfect of contaminated water with Escherichia coli was investigate...
متن کاملPreparation of Nitrogen-Doped Graphene By Solvothermal Process as Supporting Material for Fuel Cell Catalysts
Development of efficient electrocatalysts for oxygen reduction reaction (ORR) is one of the most important issues for optimizing the performance of fuel cells and metal-air batteries. The introduction of nitrogen into carbon nanostructures has created new pathways for the development of non-precious electrocatalysts in fuel cells. In this work, nitrogen-doped graphene (NG) was synthesized by a ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of cell science
دوره 7 3 شماره
صفحات -
تاریخ انتشار 1970